Fig. 4

Cadmium disrupts snRNA 3′ processing. Fold change in total or polyadenylated (Poly-A) U1 snRNA (F08H9.10), U2 snRNA (C47F8.9), U4 snRNA (C52E4.8), U5 snRNA (C53D6.16), and U6 snRNA (F35C11.9) in N2 worms measured with qPCR. Worms were fed control, ints-2, ints-4, ints-5, ints-7, or ints-8 dsRNA clones in a or exposed to 0, 100, 200, and 300 μM cadmium in b. Random or oligo-dT primers were used to synthesize total or polyadenylated cDNA from each total RNA sample. N = 4 replicates of 200–300 worms. a *P < 0.05, **P < 0.01, or ***P < 0.001 compared to control dsRNA. b *P < 0.05, **P < 0.01, or ***P < 0.001 compared to 0 μM cadmium and ^†P < 0.001 compared to the total transcript levels of the same cadmium concentration as determined by two-way ANOVA with Bonferroni post hoc tests