Synthetic Biology Goes Cell-Free

Table 1 Examples of potential therapeutics expressed in CFS to date

Therapeutic proteins	Granulocyte macrophage colony-stimulating factor (GM-CSF) [68, 70]
	Erythropoietin [70,71,72]
	Cytotoxic protein onconase [73]
	Antibodies [51, 74, 75] and antibody fragments [49, 76,77,78,79]
	Bispecific antibodies [80]
	Antibody-drug conjugates [49, 81]
	Tissue-type plasminogen activator [82,83,84,85]
Vaccine antigens	Picornaviral capsid intermediate structures [86]
	Trimeric influenza hemagglutinin head [87] and stem [88] proteins
	Trivalent vaccine based on Hc fragments of botulinum toxins A, B, and E [89]
	Anthrax protective antigen and diphtheria toxoid [49]
Virus-like particles	A B-cell lymphoma vaccine [90]
	Anti-hepatitis B VLPs [91]
	A virus-like nanoparticle scaffold for vaccines and drug delivery [92]
Antimicrobials	Antimicrobial peptides [49, 93]
Antimicrobials	Small molecules such as violacein [49, 56]

ISSN: 1741-7007