Fig. 4
From: Genetic background mutations drive neural circuit hyperconnectivity in a fragile X syndrome model
The GFI axonal projections contain chemical synapse markers. a The giant fiber interneuron (GFI) axonal bend co-labeled with 91H05-Gal4 driven mCD8::RFP (magenta, column 1) and STaR transgenic labeling of Bruchpilot (Brp) in presynaptic active zones (green, column 2). The merge reveals axonal projections with chemical synapses (column 3). Background controls (w1118, top) and dfmr1 null mutants (dfmr150M, bottom) assayed at 1 h, 1 day, and 1 week post-eclosion. Insets show magnified Brp-positive axon projections. Scale bars, 10 μm (full image) and 2 μm (inset). b Quantification of total (magenta) and Brp-positive (green) projections for all 3 time points. Significance bars represent comparisons between each genotype for the two projection quantifications for each time point. Sample sizes: 1 h: w1118, n = 17; dfmr150M, n = 12; 1 day: w1118, n = 12; dfmr150M, n = 13; 1 week: w1118, n = 8; dfmr150M, n = 8