Fig. 3
From: Mechanical force-induced morphology changes in a human fungal pathogen
The filament tip shape is not substantially altered upon invasive growth and burst out. a Filament buckling and release upon invasive growth and penetration. Time-lapse experiment at PDMS to cross-linker ratio of 40:1 (Young’s modulus ~ 100 kPa) with DIC images acquired every 2 min. b, c Filament tip shape does not substantially change during PDMS burst out. b Typical time-lapse experiment at 40:1 PDMS to cross-linker ratio (Young’s modulus ~ 100 kPa) with DIC images every 5 min. Red arrowheads indicate filament buckling. c Close up of sum projections of 13 × 0.5 μm z-sections GFP-CtRac1 fluorescent images from 3B (top), with tip curvature (bottom) at indicated times and ± 45° curvature indicated by open lines and ± 90° indicated by solid lines. d The radius of the curvature of invasive and surface growing cells is indistinguishable. The radius of the curvature is the average of 12–24 × 5 min time points (n = 11–16 cells) from 3 to 4 independent experiments for surface (PDMS ratio 40:1; Young’s modulus ~ 100 kPa) or invasively growing cells (with PDMS ratio 35:1–40:1; Young’s modulus 150–100 kPa). Bars indicate SD