Fig. 2

The second confirmation assay—quantitative serial dilution drop test. a Raw data drop test dilution validation assay. Strains were diluted to the same cell density (OD₆₁₀~1) and then further diluted in 5 sequential 10-fold dilutions, and spotted on agar. Plates that were either exposed to light or kept in the dark were imaged daily. Images of the dark control and the light exposed plates after 2 days of incubation, exemplified by wild type, yap1∆, and nsr1∆. b Growth curves of the strains shown in a where the cell-spot intensity has been estimated for images taken over 6 days. c Ranked list of the most light-sensitive strains (Student t test; p < 0.05). Values represent averages from two independent experiments, and error bars indicate standard deviation (SD) (see Additional file 5 for individual values). Blue bars denote mutants displaying a statistically significant growth difference compared to the wild-type (p < 0.05) whereas gray bars indicate non-significant growth differences. The most light-sensitive mutants confirmed in the second confirmation assays show extensive overlap with mutants sensitive to oxidative stress (red names), the HOG MAPK pathway (blue names), and diphthamide modification of eEF2 (green names). The description of each of the gene deletions can be found in Table 1