Fig. 3
From: Multi-omic profiling of pituitary thyrotropic cells and progenitors
Comparison of POU1F1 binding sites in GHFT1 and TαT1 cells. a Pou1f1 is expressed in both cell lines, contains active chromatin marks, and exhibits similar POU1F1 binding to enhancer elements. Data from RNA-seq, CUT&RUN for POU1F1 and active chromatin marks (H3K27Ac, H3K4Me1), ATAC-seq, and CUT&RUN for repressive marks (H3K27Me3) is visualized in genome browser tracks for TαT1 cells (red) and GHF-T1 (blue). b Tcf7l1 is an example of a gene uniquely bound by POU1F1 and expressed in GHF-T1 cells. c Nrxn1 is an example of a gene uniquely bound by POU1F1 and expressed in TαT1 cells. d A Venn diagram illustrating the number of shared and distinct POU1F1 binding sites throughout the genomes of GHF-T1 and TαT1 cells. e A Venn diagram showing the number of shared and distinct genes whose promoters and regulatory elements are bound by POU1F1 in GHF-T1 and TαT1 cells. f Scatter plot showing the FPKM of genes whose promoters are bound or not bound by POU1F1 in both TαT1 and GHF-T1 cells. In both TαT1 and GHF-T1 cells, genes whose promoters are bound by POU1F1 are more highly expressed. The boxed regions represent the middle quartiles, i.e., 25–75%. The significance is represented by asterisks (p values 4 × 10− 114 and 8 × 10− 130, respectively). N = 1 for POU1F1/cell line