Fig. 2

Laminin localises to cell–cell adhesions. a Confocal images of central and basal planes of WT monolayers in 2-mM Ca²⁺ fixed and stained for laminin α1, collagen IV or fibronectin. b Confocal slices of apical, central and basal planes of WT monolayers in 2mM Ca²⁺ stained for laminin α1 and F-actin or E-cadherin. c Images of the apical plane and x-z images of DMSO- and BTT (20 μm, 1h)-treated monolayers in 2-mM Ca²⁺ fixed and stained for laminin α1 and E-cadherin. d Western blot of laminin β1 in DMSO- and BTT-treated keratinocytes. e Basal laminin β1 intensities and quantification. Data are means −/+ SEM from at least 30 images per condition over 3 independent experiments; *p<0.05. Scale bars, 10μm throughout