Fig. 7.

Co-expression of brain patterning genes in the developing brain of L. ruber. A Morphology of the brain in 25-day-old juveniles. B–G Co-expression in the brain of 25-day-old juveniles. H–M Co-expression in the brain of 42-day-old juveniles. Fluorescent signal from antibody staining against tyrosinated tubulin is in yellow and from RNA probes is in magenta and green. For each panel, the color-coded names of hybridized genes are shown in the white box above the micrographs. White and red arrowheads indicate exclusive expression of one of the hybridized genes, blue arrowheads indicate co-expression. All animals are shown in dorso-ventral projection with anterior to the top; the letter in the top-right corner of each panel indicates whether the focus is on dorsal (d) or ventral (v) structures. Micrographs on B–M are not to the scale. co, cerebral organ; dbc, dorsal brain commissure; lnc, lateral nerve cord; pb, proboscis rudiment; ph, pharynx