Fig. 6
Disruption of nuclear-cytoplasmic compartmentalization in ES1 cells. a Schematic illustration of nuclear-cytoplasmic compartmentalization (NCC) reporter construct (top). 4RD-YFP tau reporter (Ctrl) and ES1 cells transiently transfected with the NCC reporter (bottom). Control cells exhibited segregated fluorescent signals, green cytoplasm and red nuclei (colored in magenta), whereas red fluorescence signals were colocalized with cytoplasmic green fluorescence in ES1 cells, indicating a defect in NCC. CV, the coefficient of variation of the mean pixel intensity of red fluorescence; RFP, red fluorescent protein; GFP, green fluorescent protein. Scale bar, 10 μm. b, c The mean pixel intensity of RFP (b) and the CV (c) in the transfected reporter (n = 71) and ES1 cells (n = 48). Cells from 8 different areas, respectively (160 μm2 for each, see Supplementary Figure 7). a.u., arbitrary unit. Scale bar, 10 μm