Table 1 Enzymatic activity of selected PACs

bPAC(wt)-eYFP

0.057 ± 0.015

93 ± 9

1630

Venus-bPAC(wt)

0.055 ± 0.01

91 ± 8

1650

EuPACα-eYFP

0.38 ± 0.06

67.6 ± 9.1

180

eYFP-EuPACα

0.35 ± 0.08

11.9 ± 1.6

34

mPAC-eYFP

1.55 ± 0.4

43.3 ± 5.5

28

LiPAC-eYFP

< 0.0005

< 0.0005

-

eYFP-LiPAC

< 0.0005

< 0.0005

-

TpPAC-eYFP

0.033 ± 0.006

5 ± 0.6

151

eYFP-TpPAC

0.04 ± 0.007

4 ± 0.4

100

bPAC(K197A)-eYFP

0.0066 ± 0.0006

2.6 ± 0.5

390

bPAC(R278A)-eYFP

0.0012 ± 0.0003

1.6 ± 0.3

1330

Venus-bPAC(S27A)

0.023 ± 0.004

94.3 ± 5.9

4100

Venus-bPAC(F198Y)

≤ 0.0024

17 ± 0.8

7080

CD8-Venus-bPAC(wt)

0.011 ± 0.0015

16.8 ± 2.0

1530

CD8-Venus-bPAC(R278A)

< 0.0005

0.52 ± 0.17

> 1000

Glyco-Venus-bPAC(wt)

0.0076 ± 0.0012

17.5 ± 3.4

2300

Glyco-Venus-bPAC(S27A)

0.0051 ± 0.0002

15 ± 0.6

2930

Glyco-Venus-bPAC(R278A)

< 0.0005

0.51 ± 0.05

> 1000

Lyn-Venus-bPAC(wt)

0.014 ± 0.003

18.2 ± 2.3

1300

2xLyn-Venus-bPAC(wt)

0.013 ± 0.003

16.9 ± 2.4

1300

2xLyn-Venus-bPAC(S27A)

0.0067 ± 0.002

18.3 ± 2.5

2730

*2xLyn-Venus-bPAC(F198Y)

< 0.0005

2 ± 0.3

> 4000