Satellitome comparison of two oedipodine grasshoppers highlights the contingent nature of satellite DNA evolution

Table 4 Stepwise multiple regression of CTR, estimated from 14 orthologous pairs of satDNA families, on four satellitome features of L. migratoria (Lmi) and O. decorus (Ode)

Item	Redundancy r^2	VIF	Step	Multiple r	Multiple r^2	r^2 increase	F	P	Partial r
Lmi_subfam	0.668	3.01
Lmi_peak_copies	0.262	1.36
Lmi_TSI	0.331	1.50
Lmi_RPS	0.236	1.31
Ode_subfam	0.118	1.13	1	0.751	0.564	0.564	15.54	0.0020	0.845
Ode_peak_copies	0.067	1.07	2	0.906	0.822	0.257	15.89	0.0021	0.758
Ode_TSI	0.172	1.21	3	0.922	0.850	0.028	1.89	0.1995	0.398
Ode_RPS	0.565	2.30

In each species, the independent variables employed were the number of subfamilies (subfam), the number of repeat units included in amplification peaks (peak_copies), the tandem structure index (TSI) and the homogenization index (RPS). Note that only three independent variables entered in the model, all of them corresponding to Ode, and only two (Ode_subfam and Ode_peak_copies) were associated with significant increases in explained variance in CTR (56.4% and 25.7%, respectively. The multiple correlation coefficients were 0.652 (SE= 0.13) and 0.466 (SE= 0.127), respectively. The Shapiro-Wilks test showed that the standardized residuals of this regression fitted a normal distribution (W= 0.966, P= 0.821). VIF= Variance inflation factors. Redundancy r^2 was performed between each independent item and the seven remaining, in order to calculate VIF as 1/(1-r^2)

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