Fig. 2

Long RNA transcripts in EVs are full-length and differ from cells. A Overview of LoTEVA pipeline for EV RNA-Seq analysis. B mRNA abundance from RNA-Seq in EVs and cells. Genes indicated by name were validated by qRT-PCR. TPM = transcripts per million. TPM values are averaged across 3 replicates. C lncRNA abundance from RNA-Seq in EVs and cells. TPM = transcripts per million. TPM values are averaged across 3 replicates. D Fold difference determined by qRT-PCR from 3 independent samples for select EV-enriched and EV-depleted transcripts. Individual values can be found in Additional file 17. E RNA-Seq read coverage of mRNA transcripts in EVs and cells. N = 3. F RNA-Seq read coverage of lncRNA transcripts in EVs and cells. N = 3. G RNA-Seq read coverage (top) and RT-PCR amplicons (bottom) of EEF1A1 mRNA. NT = no template, no-RT = RNA without reverse transcriptase. H RNA-Seq read coverage (top) and RT-PCR amplicons (bottom) of lncRNA SNHG5. NT = no template, no-RT = RNA without reverse transcriptase. Uncropped images of gels can be found in Additional file 18