Fig. 3

EV-enriched and EV-depleted transcripts have different characteristics. A Gene Ontology analysis of EV-enriched protein-coding genes performed with DAVID. For each GO category the ten significant (FDR < 0.05) terms with the lowest p-values are displayed. Individual values can be found in Additional file 17. B Broad categories of long noncoding RNAs enriched or depleted in EVs. Gray = long intergenic noncoding RNA, light blue = mRNA-associated lncRNA (antisense, intronic, or overlapping), navy = pseudogene. Individual values can be found in Additional file 17. C Specific gene biotypes of long noncoding RNAs enriched or depleted in EVs. Individual values can be found in Additional file 17. D Violin plot of transcript length for EV-enriched and EV-depleted genes. E Violin plot of number of exons per kilobase of transcript in EV-enriched and EV-depleted genes. F Violin plot of transcript half-life in actinomycin-D-treated HeLa cells for EV-enriched and EV-depleted transcripts. For all violin plots, medians are indicated above each violin and grey dotted lines indicate median of all expressed genes. P-values calculated by Welch two-sample t-test are indicated. All analyses were performed using 3 EV and 3 cell samples