Fig. 5
From: Assignment of unimodal probability distribution models for quantitative morphological phenotyping
Morphological phenotyping of yeast non-essential mutants. A Histograms showing cell sizes at the M stage (C101_C); 4708 mutants and 109 his3 replicates are shown in black and yellow, respectively. Dashed lines indicate significant thresholds for the UNIMO (purple) and Box–Cox transformed (orange) methods at FDR = 0.01. Example microscopy images of yeast cells are shown: actin, cell wall, and nucleus are shown in red, green, and blue, respectively. White arrows point to cells detected in the M stage by CalMorph. The unit for size was the number of pixels squared; for details, see the CalMorph user manual. B Yeast non-essential genes important for morphology are identified by applying UNIMO and Box–Cox transformation