Fig. 3From: ASC proneural factors are necessary for chromatin remodeling during neuroectodermal to neuroblast fate transition to ensure the timely initiation of the neural stem cell programProneural mediated chromatin changes correlate with transcriptional output during early neurogenesis. A A schematic representation of the strategy used to generate H3K27Ac ChIP-seq datasets and RNA-seq profiling. B Heatmaps of H3K27Ac ChIP-seq signal centered on Class I and Class II proneural peaks. C Genomic snapshots at the nvy and wor loci. D Boxplots of normalized H3K27Ac signal in stage 9 DHS sites from modENCODE. 2090 DHSs with proneural binding (left), not proneural-bound DHS 14,127 (right). Statistics performed with Wilcoxon rank-sum tests. E Differentially expressed genes from RNA-seq in scAPAA versus NΔE embryos FDR 0.2 (n = 4). F Gene Set Enrichment Analysis (GSEA) of RNA-seq data reveal enrichment for BDGP “VNC neuroblasts” with scAPAA>NΔΕ genes and “ventral epidermis” classes with the scAPAA<NΔΕ genes. G GSEA of differentially acetylated st.9 DHSs in scAPAA. vs. NΔΕ embryos (scAPAA> NΔΕ left, scAPAA<NΔΕ (right) with the ranked genes from the RNA-seq of the same comparison. H A Venn diagram of the GSEA Core Enrichment Genes from G (left panel) with potential target genes of the proneural consensus binding events. H List of selected 40 genes from the intersection in H with proneural binding and combined RNA-seq and differential acetylation. Differential acetylation testing on stage 9 DHS regions in wt bibG4, bib>UscAPAA, and U-NΔE embryos (n = 2) is provided in Additional file 2: Table S4. RNA-seq edgeR analysis output provided in Additional file 2: Table S5Back to article page