Fig. 5

JIP1 deficiency regulates axonal outgrowth via Notch1 intracellular domain. A Western blot analysis of Notch1 in total cell lysate from JIP1 WT and KO neurons at DIV 3. Graph represents quantification of Notch1 intracellular domain (~100kDa, Notch1-IC) levels in JIP1 WT and KO (n=6 each). Data presented as mean ± SEM and analyzed by Student’s t-test. “n” equals the number of animals. * p < 0.05. B Total cell lysate from p35 WT and KO neurons at DIV 3 subjected to western blot analysis with anti-Notch1 antibody. Quantification of Notch1 intracellular domain (Notch1-IC) levels in p35 WT (n=6) and KO (n=7) is presented as mean ± SEM and analyzed by Student’s t-test. “n” equals the number of animals. ** p < 0.01. C Representative images of JIP1 WT and KO neurons infected with adenovirus carrying GFP or Numb. D Data was analyzed by two-way ANOVA and presented as mean ± SEM; n=163 for GFP, n=132 for Numb for JIP1 WT neurons and n=248 for GFP, n=247 for Numb for JIP1 KO neurons. “n” equals the number of neurons from one experiment. **** p < 0.0001. E JIP1 WT and KO neurons immunoprecipitated with Notch1 antibody followed by immunoblotting with ubiquitin (Ub) antibody