Fig. 3

Sperm from Rnf133 KO males and Rnf133/Rnf148 DKO males demonstrate fertilization and motility defects. A Quantification of pronucleus-positive or two-cell embryos from IVF using sperm from Rnf133 HET and KO males and oocyte from WT females. n = 3 mice/genotype, and the data are expressed as the mean ± SEM. B Quantification of pronucleus-positive or two-cell embryos from in vivo fertilization by mating Rnf133 HET and KO males to WT females each. n = 3 mice/genotype, and the data are expressed as the mean ± SEM. A,B Individual data values for each replicate are provided in Additional file 18: Raw data. C Average sperm number from caudal epididymis of Rnf133 HET and KO male mice. D Total motile sperm rate measured by CASA for Rnf133 HET and KO sperm. E Progressive motility rate measured by CASA for Rnf133 HET and KO sperm. F Sperm kinetics after 15 min (left) and 120 min (right) incubation in capacitation medium for Rnf133 HET and KO sperm. VAP, average path velocity; VCL, curvilinear velocity; VSL, straight-line velocity. C,D n = 6 mice/genotype, and the data are expressed as the mean ± SEM. G Hyperactivated sperm rate classified by CASAnova after 15 min and 120 min incubation in capacitation medium for Rnf133 HET and KO sperm. n ≥ 8 mice/genotype, and the data are expressed as the mean ± SEM