Fig. 3

NvCoREST is low in proliferating cells and high in differentiated neural cells. A Confocal images of planula incubated with EdU for 30 min followed by immunofluorescence staining and Click-iT EdU detection. NvCoREST is shown in green, Click-iT EdU in magenta, and DNA in blue. B–D Confocal images of planula-stage transgenics stained for mOrange (magenta), NvCoREST (green), and DNA (blue) Transgenic lines used are indicated on the left. Stainings were performed two times independently with a minimum of 10 embryos imaged per genotype, per replicate with the same results. Scale bars: 10 µm