Fig. 5

Loss of NvCoREST leads to a defect in cnidocyte differentiation. A–D Confocal images of control or mutant primary polyps from NvCoREST Mutant 1 or 2 showing cnidocysts (green) and DNA (blue). The mutant line is shown on top and the genotype to the left. Stainings were performed three times independently with the same results with a minimum of 10 embryos imaged per condition, per replicate. E, F Confocal images of immunofluorescence staining on mutant primary polyps from NvCoREST Mutant 1 line showing mosaic patches of NvPOU4::H2B-mCherry (E) or NvPOU4::NvCoREST-mCherry (F) expression stained with anti-DsRed antibody for mCherry (magenta), DAPI for cnidocysts (green), and DNA (blue). Data was collected across two independent biological replicates and n = 10 for NvPOU4::H2B-mCherry and n = 19 for NvPOU4::NvCoREST-mCherry of which 10/10 and 18/19, respectively, had the observed phenotype. Scale bars: 50 µm