Fig. 4

Full NR7 can homodimerize on DNA. A SEC-nMS analysis of full-length NR7 and DR0 response element. The upper panel corresponds to the SEC-UV chromatogram where the chromatographic peak of the homodimer of NR7 bound to DR0 is colored in blue. The lower panel corresponds to the mass spectrum extracted from the chromatographic peak colored in blue where the homodimer of NR7 bound to DR0 is identified as the main species with a mass of 102 405 ± 1 Da (charge states are given in blue.) B SEC-MALLS analysis of full-length NR7 in the absence and presence of DR0 response elements showing the elution profile on a SEC S200 10/300 (GE healthcare) with the direct molar mass measurement of each elution peak. NR7 elutes as a dimer when bound to DR0 with a measured molar mass of around 100 kDa and as a monomer in the absence of DNA with a measured molar mass of 43 kDa. C Polyacrylamide native gel of full-length NR7 with different ratios of the DR0 response element, showing the formation of an NR7:DR0 complex, as indicated on the left by the illustration