Fig. 1

The workflow and general performance of SHERRY2 on single-cell RNA-seq. A The workflow of SHERRY2 for scRNA-seq. Poly(A) tailed RNA is firstly released from single cells and reverse transcribed. The resulting RNA/cDNA hetero-duplex is then tagmented by Tn5 transposome, followed by gap repair and indexed PCR. Optionally, chromatin can be digested during lysis. The entire protocol is performed in one tube and takes 3 h. B Gene number (FPKM > 1) with SmartSeq2, SHERRY2, and SHERRY when subsampling reads to 0.1, 0.2, 0.4, 0.6, 0.8, and 1 million reads. C Pairwise correlation of gene expression within replicates for the three scRNA-seq protocols. The correlation R-value was calculated by a linear fitting model with normalized counts of overlapped genes. D Gene body coverage detected by the three scRNA-seq protocols. The gray region represents the standard deviation of the normalized depth among replicates. E Components of reads that were mapped to different regions of the genome using the three scRNA-seq protocols. The error bars show the standard deviation. F Gene expression correlation between single HEK293T cells and 200-ng RNA extracted from HEK293T cells. Single-cell data were acquired by the three scRNA-seq protocols. Bulk RNA results were acquired by the standard NEBNext protocol. The correlation R-value was calculated by a linear fitting model with normalized gene counts. The samples in B–F are single HEK293T cells. The p-values in B, C, and F were calculated by the Mann-Whitney U test