Fig. 3

Mouse germinal center profiled by scRNA-seq through SHERRY2. A Clustering of single B cells from murine GC light zones visualized by UMAP plot. The library was prepared by SHERRY2 (with AG DNase I and DNA carrier). Different colors indicate distinct cell types. B Cell cycle and marker gene expression of different cell types marked on a UMAP plot. The gradient colors correspond to the normalized counts of a specific gene ranging from 0 (white) to 1 (blue). C Distribution of Myc gene expression in different cell types. Different colors indicate different intervals of normalized Myc counts. The percentages of cells within the clusters falling into corresponding intervals were counted. D Dynamic process of the GC light zone indicated by vector fields of RNA velocity on a UMAP plot. The expanded region shows the velocity vector of each cell. The colors correspond to the same cell types as annotated in A. E Isoforms of the Hnrnpab gene. The top two lines show isoforms from two example cells that rarely and preferentially used the highlighted exon in Hnrnpab transcripts. The bottom two lines show the isoform structures of Hnrnpab transcripts that include or exclude the exon. F Inclusion ratio distribution of the highlighted exon in E in different cell types. Only cell types represented by more than 10 cells after filtering are shown