Fig. 11

Reduced osi levels alters the storage lipid content and composition. A Assessment of total TAG content shows no significant differences at 48h but a clear decrease at 72h in mutant animals compared to controls. B For clarity purposes, TAGs with same carbon number but different degree of saturation were grouped together. TAG groups are differentially affected. The differential profile of TAG groups at 48 and 72h show that osi^100B mutants have increased low molecular weight TAG groups (additive carbon number 38 and 40), and a corresponding decrease in high molecular weight TAG groups (additive carbon number 50, 52, trend also 48). Three independent samples were analyzed. Each sample included 50 larvae pooled together. All panels: different letters indicate significant differences, p<0.05; treatments sharing any letter are not statistically different. A Shapiro-Wilk test was used for normality assessment; if normality was confirmed, a one-way ANOVA with Bonferroni’s multiple comparisons was performed. For nonparametric assessment, Kruskal-Wallis with Dunn’s multiple comparisons test were performed (see Additional file 2). The “#” symbol in last panel for 50:X group represents a strong tendency (p= 0.0523). The total number of samples analyzed is included below the corresponding panel. All datasets and statistical analysis on which the conclusions are based are included in Additional file 2