Fig. 3

Histone modifications in E. diaphana correlate with gene expression. A Distribution of histone modifications around genes with increasing expression levels. Red line marking the lowest gene expression category (RPKM < 1), and darkest blue the highest expression category (RPKM >250). B Boxplots of histone peak heights from promoter and gene body regions of methylated (dark pink) and unmethylated (light pink) genes. Genes with expression below the 30th percentile of RPKM were classified as lowly expressed, those between the 30th and 70th percentile as intermediately expressed, and those above the 70th percentile as highly expressed. (unpaired two-tailed Student’s t test; ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001). C mCpG distribution around genes with increasing gene expression levels