Fig. 2

A food-dependent behavioral switch during dauer exit. a Two-dimensional probability distributions of speed (mm/s) and angular velocity (degrees/s) averaged over 10 s time windows across the first 12 h of dauer exit, highlighting the effect of a food source on behavior. Data is from n = 3 experiments and each time point contains data from at least 250 tracks (average number of tracks = 1262). b Two-dimensional probability distributions obtained from daf-2 dauers on +OP50 NGM plates, n = 2 experiments. c Dauer behavior before and after the food-dependent behavioral switch. Shown are 10 randomly selected example tracks of a length between 2 and 4 min. d Mean speed (calculated per track) at 12 h.a.t.. *** indicates p-value < 0.0001 (Mann-Whitney test). e Schematic summarizing how the dispersal phenotype was defined, based on thresholds for speed and angular velocity (see Methods). f Relative abundance of dispersal behavior as defined in e. Error bars depict standard deviation of at least 2 experiments. g Percentage of dauers that survive SDS treatment at different time points during dauer exit. This assay can be used as a proxy for inferring onset of food ingestion. Dots represent experimental replicates