Fig. 4
From: Integrative single-cell RNA-seq and ATAC-seq analysis of myogenic differentiation in pig
Single-cell chromatin accessibility analysis of pig myogenic cells. A The myogenic cells in the scATAC-seq dataset are shown in the Uniform Manifold Approximation and Projection (UMAP) space, colored by cluster. B Top: Bar plot showing the average accessibility of 13 selected marker genes from our scRNA-seq data considering all myogenic cells. Bottom: dot plot of the standardized accessibility of the marker genes (gene body ± 2 kb) in each of the seven clusters. For each gene, the minimum accessibility value is subtracted, and the result is divided by its maximum accessibility value. The dot size indicates the percentage of cells in each cluster in which the gene of interest is accessible. The standardized accessibility level is indicated by color intensity. C UMAP visualization of the myogenic cells in the scATAC-seq dataset, colored by cell identity. D Percentage distribution of open chromatin elements in each scATAC-seq sample. E Percentage distribution of open chromatin elements in scATAC-seq myogenic cell types. F Heatmap showing cell type-specific differentially accessible peaks (DAPs) (yellow: open chromatin, purple: closed chromatin). G Distribution of open chromatin elements among DAPs in myogenic cell types. H Number of shared and unique peaks among snATAC-seq cell types