Fig. 5

Gene-family evolution in Euglenozoa. A maximum-likelihood phylogenetic tree based on the concatenated alignment of 167 proteins containing 57,565 amino acid positions. Nodes with maximal statistical support are indicated with black circles, for the remaining nodes the supports are in grey in the following format: bootstrap support/SH-aLRT value. Double-crossed branches were reduced to half of their original length. The black horizontal bar indicates the number of substitutions per site. The number of gene families lost, gained, expanded, and contracted at selected nodes (based on the sum of probabilities of the respective events at each node/tip) is indicated by the width of blue, red, orange, and green boxes, respectively. Key, event scales. Note the exceptionally large gain and expansion of more than 7000 and 1400 gene families, respectively, on the ancestral diplonemid branch. Diplonemid, kinetoplastid, and euglenid taxa are highlighted in beige background shades. LDCA, last diplonemid common ancestor