Fig. 7

Dgd2p functional region determination. A Up, a general view of the positions of the identified functional sites in the CR85 Dgd2p compared to the other Dgd2p. Down, alignment of the available Saccharomyces Dgd2p amino acid sequences deduced from the orthologous nucleotide sequence, using CR85 as a reference. The upper green lines of each sequence indicate the encoded Dgd2p sequences according to the gene annotations. It can be observed that in most strains the methionines of the start codons are located 60–74 codons downstream of the start codon of CR85. When this homologous region of 60–74 codons is translated in the other strains, the start codon of CR85 is absent, and stop codons appear, indicating that this region is pseudogenized in all strains except CR85. B Alignment of the middle homology region (MHR) of S. cerevisiae zinc binuclear proteins with the CR85 Dgd2p sequence. (*) The conserved MHR amino acids were described elsewhere [35]. C Alignment of the S. cerevisiae Cha4p activation domain (AD) with the CR85 Dgd1p C-terminal region. D Identity of each Saccharomyces Dgd2p and the consensus amino acid sequence with respect to S. cerevisiae Cha4p. The numbers in the brackets indicate the amino acid positions of Chap4p referred to the start codon of CR85 Dgd2p