Fig. 4
GhBM/GbBM directly targeting its own promoter. A, B Transient transcriptional activity analysis of Beauty Mark using luciferase transient expression. Transient expression assays showing that GhBM repress the transcriptional activity of both GhBMPro:LUC and GbBMPro:LUC (A). Representative images of N. benthamiana leaves 72 h after infiltration are shown. The left panel indicates the infiltrated constructs. Scale bar = 0.5 cm. Quantitative analysis of luminescence intensity with different letters indicates significant differences (P ≤ 0.05, one-way ANOVA, Tukey’s HSD test) (B). C AD-BM activates the expression of the LacZ reporter genes driven by the promoters of respective GhBM and GbBM in yeast. Representative data are shown from one of three biological replicates, which yielded similar results. D The regions tested by ChIP assays are shown in the schematic representation. The putative MYB-core elements in the promoter of GhBM/GbBM gene are indicated by black lines. E ChIP assays indicating the association of GbBM/GhBM with several regions in the promoters of GbBM and GhBM. The promoter of GbActin1 was used for normalization. Bars represent means SD of three biological replicates. Student t-test was used to generate P value