Fig. 4

Immunodetection of Aristaless1 in white and yellow Heliconius cydno pupal forewings. A Adult forewing of a white Heliconius cydno. B Al1 detection in a full pupal wing of a white Heliconius cydno (3 days APF). C Details of Al1, DNA, and actin detection in precursor scale cells of future melanic scales from a white Heliconius cydno. D Al1 detection in precursor scale cells of future white scales. E Side digital reconstruction from z-stack showing Al1 within precursor scale cells from the white part of the wing. F Adult forewing of a yellow Heliconius cydno. G Al1 detection in a full pupal wing of a yellow Heliconius cydno. H Details of Al1 detection in precursor scale cells of future melanic scales from a yellow Heliconius cydno. I Al1 detection in precursor scale cells of future yellows scales. J–K Side digital reconstruction from z-stack showing differences in Al1 detection within precursor scale cells from yellow and melanic portions of a yellow Heliconius cydno wing. Panels show detection of DNA (C,D,E,H,I), F-actin (C',D',H',I’), Al1 (C'',D'', E’, H’’,I’’), F-actin/DNA (B, G), and a merge (C''', D''',E’’,H'',I’’’,J-K) view. Scale bars: B, G are 1000 μm; C–E and H-I are 100 μm; J–K are 50 μm