Fig. 1
From: Egg-laying by female Aedes aegypti shapes the bacterial communities of breeding sites
Detection of profile shifts on microbial communities induced by oviposition-related inputs. An experimental scheme was elaborated to dissect the effects of the act of oviposition from other sources affecting microbial profiles observed after mosquito-water interactions. DNA extracted from water samples belonging to five different treatments was subjected to amplicon-oriented sequencing to characterize the structure of their bacterial communities. Treatment 1 (T1): environmental control (type I water plus sterilized food). Treatment 2 (T2): manually-deposited sterilized mosquito eggs. Treatment 3 (T3): manually-deposited non-sterilized eggs. Treatment 4 (T4): single sugar-fed females interacting with container water. Treatment 5 (T5): single gravid female (72Â h post-blood-feeding) allowed to lay eggs