Fig. 7

Spatial transcriptome sequencing deciphers the molecular variations during tendinopathy progression. A UMAP visualization of the distribution of cells at different sample statuses (left). UMAP visualization of 10 cell clusters in spatial transcriptome sample (right). B The proportion of each subcluster in the normal and diseased groups. C H&E staining of normal and diseased tendons (left) and distribution of 10 clusters in the tissue space (right). D Dis-TC score of 10 clusters in the diseased group (left). Spatial heatmaps showing the Dis-TC score (middle) and the expression level of POSTN (right) in the diseased group. E Dis-TC score of 10 clusters in the normal group. Spatial heatmaps showing the Dis-TC score (middle) and the expression level of POSTN (right) in the normal group. F Mac score of 10 clusters in the diseased group. Spatial heatmaps depicting the Dis-TC score (middle) and the expression level of CD68 (right) in the diseased group. G Mac score of each cluster of the normal group. Spatial heatmaps depicting the Dis-TC score (middle) and the expression level of CD68 (right) in the normal group. H EC1 score of each cluster in the diseased group (left). Spatial heatmaps depicting the EC1 score (middle) and the expression level of CD31 (right) in the diseased group. I EC1 score of each cluster in the normal group (left). Spatial heatmaps depicting the EC1 score (middle) and the expression level of CD31 (right) in the normal group. J, K Spotlight plots showing the spatial distribution of different cell populations in normal (J) and degenerative (K) tendons