Fig. 4

Cleavage of DPCs by MUS81 in nuclear extracts. A A nicked duplex modified with streptavidin was incubated with different amounts of nuclear extracts from CAL51 MUS81-KO (lanes 2 and 3) or WT CAL51 (lanes 4 and 5) cells at 37 °C for 1 h. The reaction was also performed with recombinant MUS81-EME1 complex (2.5 nM, lane 6). The small arrow indicates an unspecific band, most likely the product of the removal of the biotin moiety from the residual oligonucleotide without streptavidin. B Quantification of the reaction products from A. Relative fluorescence units (RFU) are the arbitrary units of the imaging system for fluorescence intensity. The means and standard deviations from three independent experiments are shown. The P value (**P < 0.01) was calculated through an unpaired two-tailed t-test. Individual data values can be found in Additional file 10