Fig. 1
From: Versatile generation of precise gene edits in bovines using SEGCPN
Scheme for performing various types of precise gene editing based on embryo-specific self-excising gene targeting. a Scheme for performing various types of precise gene editing. The embryo-specific self-excising element (ESSEE) was cloned into the gene-targeting vector. BFFs identified to have modifications at the specific locus were used for SCNT to generate genetically modified blastocysts or individual animals. GI, gene of interest for precise gene editing; *, subtle mutation; TAG, gene-tagging gene; GR, gene-replacement gene; DTA, diphtheria toxin A as negative selection marker. b Scheme of nucleofection, selection, embryo reconstruction, and embryo transferation using the SEGCPN