Fig. 1
From: Protein secretion zones during overexpression of amylase within the Gram-positive cell wall
AmyE-mCherry is efficiently secreted from B. subtilis and B. licheniformis cells, but also detectable in a cell-associated from. A Western blot showing the presence of AmyE-mCherry fusion protein (calculated Mw: 99.3 kDa) in cell lysates of B. subtilis and B. licheniformis after 16 h of growth (note that duplicates are shown for assessment of reproducibility) using polyclonal antibodies against mCherry; B fluorescence measurement of the whole culture, supernatant, or separated cells; and C amylase activity in culture supernatant. AmyE, plasmid-based expression of amyE; AmyE-mCherry, plasmid-based expression of the reporter construct; WT, native genomic expression of amyE (C). ∆sp-AmyE-mCherry represents a variant without signal peptide in B