Fig. 2
From: Protein secretion zones during overexpression of amylase within the Gram-positive cell wall
Dependence of cell density and fractions of cells producing AmyE-mCherry on growth phases. A Growth curves of B. subtilis and B. licheniformis incubated at 37 °C for 21 h. B Kinetics of mCherry fluorescence of the whole culture (cell-associated plus culture supernatant) normalized to cell density. AmyE-mCherry production in B. subtilis and B. licheniformis during 10 h of growth. C Growth curves of B. subtilis and B. licheniformis incubated at 37 °C for 10 h. D Amylase activity in culture supernatant of B. subtilis and B. licheniformis. E Western blot showing the presence of AmyE-mCherry fusion protein (calculated Mw: 99.3 kDa) in cell lysates of B. subtilis and B. licheniformis. F SDS PAGE gel is shown as a loading control