Fig. 3
From: Protein secretion zones during overexpression of amylase within the Gram-positive cell wall
Fraction of cells showing AmyE-mCherry signals in different growth phases. A Structured illumination microscopy (SIM) imaging of B. subtilis (left) and B. licheniformis cells (right) showing AmyE-mCherry fluorescence during different growth phases. B Table showing percentage and number of analyzed cells. C The variant ∆sp-AmyE-mCherry without signal peptide accumulates in the cytosol. Structured illumination microscopy (SIM) imaging of B. subtilis (top) and B. licheniformis cells (bottom) showing ∆sp-AmyE-mCherry fluorescence during the transitional growth phase. (D) Western blot showing the presence of ∆sp-AmyE-mCherry (calculated Mw: 96.4 kDa) and AmyE-mCherry fusion protein (calculated Mw: 99.3 kDa) in cell lysates of B. subtilis (top) and B. licheniformis (bottom) after 16 h of growth