Table 1 SMT data from SecA-mNeonGreen

Number of cells

188

194

161

83

Average cell length [μm]

2.7

2.8

2.6

2.7

Number of tracks

18,455

11,603

11,556

3566

Population_static [%]

20.9 ± 0.001

29.0 ± 0.001

24.4 ± 0.002

29.7 ± 0.002

Population_mobile [%]

50.8 ± 0

50.4 ± 0

46.9 ± 0.001

57.0 ± 0.001

Population_free [%]

28.3 ± 0.001

20.6 ± 0.001

28.7 ± 0.002

13.0 ± 0.002

D_static [μm² s⁻¹]

0.04 ± 0

0.04 ± 0

0.03 ± 0

0.03 ± 0

D_mobile [μm² s⁻¹]

0.15 ± 0

0.15 ± 0

0.11 ± 0

0.11 ± 0

D_free [μm² s⁻¹]

0.70 ± 0.001

0.70 ± 0.001

0.60 ± 0.002

0.60 ± 0.002

R²

1

1

1

1

Average dwell time τ [s]

0.297 ± 0.004

0.301 ± 0.005

0.306 ± 0.004

0.288 ± 0.005

τ₁ (2-comp.) [s]

0.24 ± 0.002

0.23 ± 0.002

0.25 ± 0.001

0.23 ± 0.003

Fraction τ₁ [%]

78.2 ± 1.95

70.9 ± 1.98

80.7 ± 1.36

71.8 ± 4.01

τ₂ (2-comp.) [s]

0.45 ± 0.015

0.43 ± 0.012

0.48 ± 0.013

0.39 ± 0.017

Fraction τ₂ [%]

21.8 ± 1.95

29.1 ± 1.98

19.3 ± 1.36

28.2 ± 4.01