Fig. 1

scRNA-seq analysis of mouse lung ILC2s. a Experimental design for scRNA-seq of ILC2s sorted from lungs of papain-challenged mice (samples from 2 mice at each timepoint of WT or PD-1 KO mice were mixed). b A t-SNE clustering plot showing the grouped clusters of 341 lung-derived ILC2s. Each point represents an individual cell. Cells are colored by clusters. c A t-SNE plot showing the time points information about the lung ILC2. Cells are colored by different time points. d Stacked violin plots showing the distributions of expression of ILC2-associated genes. The y-axis indicates the log2 (normalized count + 1) expression levels. e A heat map shows the expression of selected differentially expressed genes for each ILC2 subset. The Wilcoxon rank sum test is used for analysis. Colors are based on normalized expression levels. f The cell composition of each cluster. Colors represent different timepoint cells. g Proportions of ILC2 subsets at different time points. Colors represent clusters. h Venn diagrams showing the upregulated genes in C1, C2, C3, and C4 respectively by pairwise comparisons (P value < 0.05, log2FC > 1). The Wilcoxon rank sum test is used for analysis. i-j The bar chart shows the GO pathways (i) and KEGG (j) analysis of DEGs (P value < 0.05, log2FC > 1) between four subsets. The colors represent the subsets. The top five terms of each subset are showed