Fig. 2

The comparison between C3 and C4 clusters. a A t-SNE plot showing the cell state of our 4 ILC2 subsets by of SingleR algorithm annotations based on 3 published mouse lung ILC2s datasets (GSE122762; GSE123994; GSE117567). Red dots indicate activated ILC2s. Blue dots indicate non-activated ILC2s. b Box plot shows the distribution of activation scores by cluster. The activation gene sets were sourced from a published study [51]. c DEGs in C4 compared with C3 are shown in the volcano plot. P < 0.01 and log2FC > 1 were set as the cut-off criterion of significant difference. Red dots indicate the upregulated genes. Blue dots indicate the downregulated genes. d-e The bar chart shows the GO terms (d) and KEGG pathways (e) analysis of DEGs (P value < 0.05 and log2FC > 1) in C4 compared with C3. f Box plot shows the distribution of wound healing scores by cluster. The wound healing score gene sets were sourced from a published report [39]. g The dot plot shows distributions of the Batf and Areg expressions among clusters. h Box plot shows the distribution of proliferation scores by cluster. The proliferation gene sets were sourced from a published study [39]. i Box plot shows the distribution of memory scores by cluster. The memory gene sets were sourced from a published study [53]. j The dot plot shows the distributions of expression of activation, wound healing, proliferation, memory, and migrant genes among clusters. All the signature gene sets can be found in Additional file 3, Table S2