Fig. 3

The identification of Nr4a1⁺ ILC2 subset. a A 3D plot displaying the distribution of 4 ILC2 clusters by diffusion mapping. DC represents the diffusion component. b The box plot shows the distribution of tissue-resident scores by cluster. The gene sets were sourced a previous study [51]. All the signature gene sets can be found in Additional file 3, Table S2. c Heatmap showing the representative Trm and Tem/Tcm related DEGs. d-e Box plot shows the distribution of Trm (d) and Tem/Tcm (e) scores by cluster. The gene sets were sourced from a previous study [53]. All the signature gene sets can be found in Additional file 3, Table S2. f The dot plot shows expressions of the Gata3, Il7r, Pdcd1, Cd69, and Nr4a1 among clusters. g Experimental design for C4 cell validation. h FACS plots showing the identification of CD45⁺Lin⁻IL-7Rα⁺IL-33R⁺CD25⁺PD-1^hiCD69^hiNr4a1⁺ ILC2 cells in the lung of Nr4a1^eGFPcre/+ mice (n = 3 mice per group). i The percentages of CD69^hiNr4a1⁺ cells in activated PD-1^hiILC2s at d0, d7, and d14. Data are presented as means ± SEM. Significances between means were analyzed by one-way ANOVA test. j Experimental design for Nr4a1⁺ILC2s validation in Il5-expressing ILC2s. k FACS plots showing the identification of CD45⁺Lin⁻IL-7Rα⁺IL-33R⁺CD25⁺IL-5⁺Nr4a1⁺ ILC2 cells in the lung of Nr4a1^eGFPcre/+Il-5^tdtomato/+ mice d7 after papain challenge