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Fig. 1 | BMC Biology

Fig. 1

From: An intelligent workflow for sub-nanoscale 3D reconstruction of intact synapses from serial section electron tomography

Fig. 1

Detail of the workflow. (I) Localization of ROI in serial-section. To locate the ROIs in the serial sections, ordered serial sections are obtained, then affine transformation is used to perform coarse-to-fine ROI localization according to the position relationship between them. (II) Alignment of serial-section. Multi-resolution coarse alignment and BP-Elastic fine alignment of serial sections are performed using the designed algorithm to obtain aligned volumes. (III) Reconstruction of volume. To obtain the intact reconstructed volume, using the designed methods to estimate and generate the missing information of all sections. (IV) Reconstruction of ultrastructures. A 3D neural network combined with manual proofreading was applied to segment synapse ultrastructures. SerialEM, IMOD, and Amira are used for image acquisition, ET reconstruction, and image rendering

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BMC Biology

ISSN: 1741-7007

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