Fig. 1

Direct association of RXRα with pre-miR-103a-2. a The in vitro transcribed pre-miR-103a-2 was incubated with Glutathione Sepharose beads together with GST or GST-RXRα. The RNA pulled down was eluted and subjected to qRT-PCR analysis to detect pre-miR-103a-2. The group of samples untreated with reverse transcriptase (-RT) but subjected to PCR analysis was a control. The PCR values normalized to the PCR value of pre-miR-103a-2 pulled down by GST were presented as the relative amount of pre-miR-103a-2. b GST pull-down analysis of the association of the in vitro transcribed pre-miR-103a-2 with GST, GST-RXRα, and GST-Smad1 proteins. c RNA-IP analysis of the association between pre-miR-103a-2 and RXRα in AD293 cells. The lysates from control and stable Flag-RXRα-expressed AD293 cells were subjected to immunoprecipitation with anti-Flag antibody or non-specific IgG, followed by qRT-PCR analysis using the specific primers for pre-miR-103a-2. The amount of pre-miR-103a-2 pulled down was expressed as a percentage of the input. d RNA-IP analysis of the association between RXRα and some pre-miRNAs in the stable Flag-RXRα-expressed AD293 cells. Data are presented as mean ± SEM (n = 3), ****P < 0.0001, and **P < 0.01