Fig. 4

RXRα inhibits the processing of pre-miR-103a-2 into miR-103. a–d HeLa cells (a,b) or MDA-MB-231 cells (c,d) were transfected with the indicated expression plasmids or siRNAs for 48 h. QRT-PCR was applied to detect the expression levels of the indicated primary (pri), precursor (pre), and mature forms of miRNAs. The values of the groups of Myc-RXRα overexpression and RXRα knockdown were normalized to their corresponding control groups, respectively. e HeLa cells were transfected with pCMV vector or pCMV-Myc-RXRα plasmid, along with pcDNA3.1-pre-miR-103a-2 plasmid and pcDNA3.1 vectors containing M2 and M5 genes, and the relative RNA levels of pre-miR-103a-2 and miR-103 were analyzed. To exclude the interference due to the transfection efficiency, neomycin gene in the pcDNA3.1 vector but not in the pCMV vector was used as an internal control for normalization. Data are presented as mean ± SEM (n = 3), ****P < 0.0001, **P < 0.01, * P < 0.05, and ns means not significant