Fig. 5

RXRα inhibits XPO5-mediated nuclear export of pre-miR-103a-2. a HeLa cells transfected with control or Myc-RXRα expression plasmids were subjected to cellular fractionation to obtain nucleus and cytoplasm parts for RNA extraction. The expression levels of pre-miR-103a-2, pre-miR-145, pre-miR103b-1 and GAPDH mRNA in nucleus and cytoplasm were quantified by qRT-PCR. b,c HeLa cells (b) or MDA-MB-231 cells (c) were transfected with control or Myc-RXRα expression plasmids. Cell lysates was used for immunoprecipitation with the anti-XPO5 antibody or non-specific IgG, and the immunoprecipitated pre-miR-103a-2 was quantified by qRT-PCR. d HeLa cells were transfected with Myc-RXRα expression plasmid along with pre-miR-103a-2 or its mutant expression plasmids. Cell lysate was used for immunoprecipitation with the anti-XPO5 antibody or non-specific IgG, and the immunoprecipitated pre-miR-103a-2 and its mutants were quantified by qRT-PCR. e A mechanistic working model of RXRα inhibiting pre-miR-103a-2 processing. Data are presented as mean ± SEM (n = 3), ****P < 0.0001, and ns means not significant