Fig. 6From: Nuclear receptor RXRα binds the precursor of miR-103 to inhibit its maturationRXRα up-regulates the expression level of Dicer. a, b The mRNA and protein levels of Dicer in MDA-MB-231 cells transfected with the indicated miRNA mimics or inhibitors were analyzed by qRT-PCR assay (a) and western blot assay (b), respectively. c, d The mRNA and protein levels of Dicer in MDA-MB-231 cells transfected with Myc-RXRα expression plasmid or RXRα siRNA (si-RXRα) were analyzed by qRT-PCR assay (c) and western blot assay (d), respectively. e,f MDA-MB-231 cells were transfected with RXRα siRNA or control siRNA along with miR-103 inhibitor or control inhibitor. The mRNA and protein levels of Dicer were analyzed by qRT-PCR assay (e) and western blot assay (f), respectively. The intensity of the bands of Dicer and β-actin was quantified by ImageJ software, and the intensity ratios of Dicer/β-actin were presented (b, d, and f). Ctrl, control. Data are presented as mean ± SEM (n = 3), ****P < 0.0001, ***P < 0.001, ** P < 0.01, and *P < 0.05Back to article page