Fig. 4

Dual gRNAs induce targeted recombination within a region that undergoes an inversion. A Schematic diagram showing two gRNAs each targeting the C. briggsae (top) and the C. nigoni genomes (bottom), respectively. Note that the syntenic genomic sequences between the two species are alignable but undergo an inversion, which prevents the recovery of any viable progeny resulting from homology-based recombination. B Confirmation of a targeted recombinant within the inverted region induced by the dual gRNAs through Nanopore DNA sequencing. Shown are sequencing reads and the gene models that span the recombination boundary. C A magnified view of the recombination boundary. The gRNA sequences and PAM sequences are highlighted in purple and green, respectively