Fig. 5

Dual gRNAs induce targeted recombination in the introgression strain. A Schematic diagram of species-specific dual gRNAs that induce DSBs in an introgression strain. For simplicity, recombinant carrying C. briggsae fragments without the GFP marker is not shown. C. briggsae and C. nigoni sequences are differentially color coded as in Fig. 1. B Comparison of targeted recombinant frequency in the progeny of introgression strain induced by a single species-specific gRNA and dual gRNAs. The number of total screened worms (n) are also indicated. Error bar represents 95% confidence interval calculated as in Fig. 2B. (∗ ∗) p < 0.01 (Fisher’s exact test with multiple testing correction using FDR method). C Confirmation of the targeted recombinant induced by the dual gRNAs through Sanger DNA sequencing. The gRNA sequences and PAM sequences are highlighted in purple and green, respectively