Fig. 5

Co-expression network of key transcription factors of FA biosynthesis and haplotypes for the gene DOF4.4 (BnaA03G0459300ZS). a Simplified regulatory network of FA biosynthesis in Arabidopsis. The blue line indicates a positive relationship. The red dashed line indicates a negative relationship. b Co-expression network of LEC1, LEC2, and WRI1. To avoid duplication, networks of different homolog genes are incorporated. Circles in orange represent acyl-lipid genes, and circles in green represent the transcription factor genes. Correlation between the expression (TPM) of BnaA03.DOF4.4 with oil content (c) and seed coat content (f). Box plots for oil content (d) and seed coat content (g) for the two haplotypes (n = 238 versus 22). e Box plots for the expression for the two haplotypes (n = 185 versus 20). Center line, median; box limits, upper and lower quartiles; whiskers, 1.5 × the interquartile range; dots, outliers (P < 2e − 16, Student’s t test)