Fig. 5
From: Multi-omic insights into the formation and evolution of a novel shell microstructure in oysters
Evolution of Pif and LamG3 in metazoans. a Maximum likelihood (ML) tree of Pif and LamG3 in 16 selected metazoans. The different colors of protein IDs represent distinct lineages. Yellow, orange, and blue solid circles before protein IDs represent Pif, ancestral Pif, and LamG3 genes, respectively. Red branches on the clades represent three groups of genes which are all highly expressed in the mantle tissue (Additional file 1: S17). Numbers on the nodes are bootstrap values (> 50%). b The evolutionary model of LamG3, Pif, and ancestral Pif. Red box represents the signal peptide (SP) domain. Blue, green, and purple boxes represent chitin binding (CB), von Willebrand factor type A (VWA), and concanavalin A-like lectin/glucanase (LG) domains, respectively. Dash box indicate potential presence of SP domain. c Reconstructions of evolution of Pif and LamG3 in metazoans. Orange boxes on the branches indicate the origin or loss of ancestral Pif genes. Yellow and blue boxes represent the events of VWA and LG domain loss, respectively